Composite

Part:BBa_K300006:Design

Designed by: Susanna Zucca, Federica Sampietro, Paolo Magni   Group: iGEM10_UNIPV-Pavia   (2010-10-04)

mOrange with ADH1 transcriptional terminator for yeast


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 763
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We used BioBrick Standard Assembly (BBa_E2050 was in RFC10 Standard, while BBa_K165018 was in RFC23 Standard).

This part has a RFC10 Prefix and a RFC23 Suffix.

Source

BBa_E2050 - from 2010 DNA Distribution;

BBa_K165018 - from 2010 DNA Distribution.

References

Shaner NC, Campbell RE, Steinbach PA, Giepmans BN, Palmer AE, Tsien RY (2004), Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nat Biotechnol. Dec;22(12):1567-72.